Journal: Cell Communication and Signaling : CCS

Article Title: Long non-coding RNA H19 transported by colorectal cancer small extracellular vesicles promotes alternative splicing in healthy hepatocytes: new insights on liver pre-metastatic niche formation

doi: 10.1186/s12964-026-02738-x

Figure Lengend Snippet: a qRT-PCR showing lncH19 levels inside CRC-sEVs and CRC_cells. b Representative images of western blot for the presence of RBFOX2 in the CRC-sEVs protein lysates. c Representative Image showing the proposed mechanism of lncH19 acting as shuttle RNA transferring RBFOX2 inside the EVs. d Representative confocal image of RNA in situ assay coupled with immunocytochemistry in the THLE2 treated for 3 h with the CRC-sEVs, lncH19 (red) and RBFOX2 (green). Scale bar:60 μm. The results reported in the graphs are the mean ± SD of three independent experiments. Statistical analyses were performed using Ordinary one-way ANOVA * p < 0.05, ** p < 0.01, *** p < 0.001. e Graphs showing the mean fluorescence intensity (MFI) relative to RBFOX2 (left) and lncH19 (right) of the different conditions

Article Snippet: The SV40 large T antigen-immortalized healthy human liver epithelial cell line THLE2 (ATCC, Manassas, VA) was cultured in Airway Epithelial Cell Basal Medium (ATCC, Manassas, VA) with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300–040, Manassas, VA) supplemented with 5 ng/ml epidermal growth factor (EGF), 70 ng/ml phosphoethanolamine, 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/ml streptomycin (Euroclone, UK) at 37 °C with 5% CO 2 .

Techniques: Quantitative RT-PCR, Western Blot, Transferring, In Situ, Immunocytochemistry, Fluorescence

Journal: Cell Communication and Signaling : CCS

Article Title: Long non-coding RNA H19 transported by colorectal cancer small extracellular vesicles promotes alternative splicing in healthy hepatocytes: new insights on liver pre-metastatic niche formation

doi: 10.1186/s12964-026-02738-x

Figure Lengend Snippet: a Representative c onfocal micrographs of RNA in situ hybridisation in THLE2 treated for 18 h with the wt-CRC-sEVs and the sh-CRC-sEVs (lncH19 in red, Hoechst in blue). b MFI analysis obtained with NIS 1 A analysis software, and ( c ) qRT-PCR showing lncH19 levels in treated cells. ( d ) Representative confocal micrographs of immunocytochemistry for RBFOX2 in the THLE2 treated with wt- and sh- sEVs for 18 h and relative Scale bar: 60 μm ( e ) MFI analysis.The results reported in the graphs are the mean ± SD of three independent experiments. Statistical analyses were performed using Ordinary one-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: The SV40 large T antigen-immortalized healthy human liver epithelial cell line THLE2 (ATCC, Manassas, VA) was cultured in Airway Epithelial Cell Basal Medium (ATCC, Manassas, VA) with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300–040, Manassas, VA) supplemented with 5 ng/ml epidermal growth factor (EGF), 70 ng/ml phosphoethanolamine, 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/ml streptomycin (Euroclone, UK) at 37 °C with 5% CO 2 .

Techniques: In Situ, Hybridization, Software, Quantitative RT-PCR, Immunocytochemistry

Journal: Cell Communication and Signaling : CCS

Article Title: Long non-coding RNA H19 transported by colorectal cancer small extracellular vesicles promotes alternative splicing in healthy hepatocytes: new insights on liver pre-metastatic niche formation

doi: 10.1186/s12964-026-02738-x

Figure Lengend Snippet: a qRT-PCR and ( b ) exon-specific qRT-PCR showing the expression levels of ENAH, CTTN and PARD3 genes in THLE2 treated with the wt- and sh-CRC-sEVs

Article Snippet: The SV40 large T antigen-immortalized healthy human liver epithelial cell line THLE2 (ATCC, Manassas, VA) was cultured in Airway Epithelial Cell Basal Medium (ATCC, Manassas, VA) with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300–040, Manassas, VA) supplemented with 5 ng/ml epidermal growth factor (EGF), 70 ng/ml phosphoethanolamine, 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/ml streptomycin (Euroclone, UK) at 37 °C with 5% CO 2 .

Techniques: Quantitative RT-PCR, Expressing

Journal: Cell Communication and Signaling : CCS

Article Title: Long non-coding RNA H19 transported by colorectal cancer small extracellular vesicles promotes alternative splicing in healthy hepatocytes: new insights on liver pre-metastatic niche formation

doi: 10.1186/s12964-026-02738-x

Figure Lengend Snippet: a Table showing the different miRNAs identified in the three different analyses (sponged by lncH19, involved in EMT and expressed by healthy liver tissue). b Venn Diagram from FunRich analysis of data obtained by the three indicated datasets. The square in figure indicates 4 from 11 miRNAs targeting PARD3 mRNA c qRT-PCR confirming the over-expression of lncH19 in the THLE2 and ( d ) the increase of PARD3 mRNA in the H19-overexpressing hepatocytes

Article Snippet: The SV40 large T antigen-immortalized healthy human liver epithelial cell line THLE2 (ATCC, Manassas, VA) was cultured in Airway Epithelial Cell Basal Medium (ATCC, Manassas, VA) with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300–040, Manassas, VA) supplemented with 5 ng/ml epidermal growth factor (EGF), 70 ng/ml phosphoethanolamine, 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/ml streptomycin (Euroclone, UK) at 37 °C with 5% CO 2 .

Techniques: Quantitative RT-PCR, Over Expression